Separase

Separase
Identifiers
EC no.	3.4.22.49
CAS no.	351527-77-0
Databases
BRENDA	enzyme data
ExPASy	NiceZyme view
KEGG	enzyme entry
MetaCyc	metabolic pathway
Rhea	reactions
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

ESPL1
Identifiers
Aliases	ESPL1, ESP1, SEPA, EPAS1, Separase, extra spindle pole bodies like 1, separase
External IDs	OMIM: 604143; MGI: 2146156; GeneCards: ESPL1
Enzyme activity EC # BRENDA ExPASy KEGG MetaCyc 3.4.22.49 ↗ ↗ ↗ ↗
Gene location (Human) Chr. Chromosome 12 (human) Band 12q13.13 Start 53,268,299 bp End 53,293,638 bp
Gene location (Mouse) Chr. Chromosome 15 (mouse) Band 15|15 F2 Start 102,204,701 bp End 102,232,792 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in secondary oocyte ventricular zone jejunal mucosa oral cavity gonad mucosa of ileum embryo gingival epithelium amniotic fluid mucosa of pharynx Top expressed in oocyte primary oocyte epiblast secondary oocyte hand ventricular zone seminiferous tubule superior cervical ganglion spermatid spermatocyte More reference expression data BioGPS More reference expression data
Gene ontology Molecular function cysteine-type peptidase activity peptidase activity catalytic activity protein binding cysteine-type endopeptidase activity hydrolase activity Cellular component cytoplasm cytosol centrosome mitotic spindle nucleus Biological process negative regulation of sister chromatid cohesion chromosome segregation homologous chromosome segregation proteolysis positive regulation of mitotic metaphase/anaphase transition meiotic chromosome separation meiotic spindle organization meiosis I mitotic sister chromatid segregation establishment of mitotic spindle localization apoptotic process mitotic cell cycle mitotic cytokinesis Sources:Amigo / QuickGO
Orthologs Databases NCBI: entry; OMA: entry Species Human Mouse Entrez 9700 105988 Ensembl ENSG00000135476 ENSMUSG00000058290 UniProt Q14674 P60330 RefSeq (mRNA) NM_012291 NM_001014976 NM_001356312 RefSeq (protein) NP_036423 NP_001014976 NP_001343241 Location (UCSC) Chr 12: 53.27 – 53.29 Mb Chr 15: 102.2 – 102.23 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Separase, also known as separin, is a cysteine protease responsible for triggering anaphase by hydrolysing cohesin, which is the protein responsible for binding sister chromatids during the early stage of anaphase. In humans, separin is encoded by the ESPL1 gene.